Regulatory

Part:BBa_K1072023:Experience

Designed by: junjie zhang   Group: iGEM13_SCUT   (2013-09-15)


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Applications of BBa_K1072023

BBa_K2368005

Introduction

Pfus

Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in S.cerevisiae, when STE2 binding with α pheromone, STE12 will stimulate FUS1 promoter, thereby initiating the mating of yeast.

However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)

标题 ‎

Fig.1 The sequencing result of Pfus

This part is an improvement of BBa_K1072023 of 2013 SCUT team.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



User Reviews

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